GPCRs Expression Profiles


Cerebellar Granule Neurones (CGN)

Genome-wide profiling of G protein-coupled receptors in cerebellar granule neurons using high-throughput, real-time PCR
B Maurel, AL Digarcher, C Dantec, L Journot. 2011. BMC Genomics. 12:241.
Various institutions in Montpellier, France.


Outcomes:

They have determined the repertoire of GPCRs expressed in cerebellar granule neurones (CGN) and neuroblasts during postnatal development.

They have newly identified tens of GPCRs which haven’t been detected previously in CGN.


Cells: Cultured murine cerebellar granule neuroblasts/neurones

The effect of endogenous glutamate, which stimulates NMDA-R, on the gene expression was minimised by NMDA antagonist MK-801 (1μM) added to the culture media.

Methods: Reverse transcription and real-time PCR.

Statistical analysis performed by MeV4.2 (TM4), also by XLSTAT (Addinsoft) for post-hoc tests for Kruskal-Wallis test.


Additional information: Primer sequences viewable in additional files.



Cell-lines: HEK293, AtT20, BV2, and N18

Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis

BK Atwood, J Lopez, J Wager-Miller, K Mackie, A Straiker. 2011. BMC Genomics. 12:14


Cells: HEK293 human embryonic kidney cell-line, AtT20 murine pituitary corticotroph tumor cells, BV2 micorglia, and N18 neuroblastoma.

BV2 was maintained towards a non-activated state.


Methods: Microarray and Quantitative PCR

Microarray data was first log2 transformed (Bono et al. 2003), then values for unlikely expressions were determined. Standard statistical analysis performed by Graph Pad Prism 4.0 (Hearne Scientific Software, Chicago, IL).


Results: The expression profiles were charted in a highly comprehensible manner in the original paper (open access).


Ref.
Bono H. et al. 2003. Systematic expression profiling of the mouse transcriptome using RIKEN cDNA microarrays. Genome Res. 13(6B):1318-1323.